Experiment information
Accession CRX124884
Organism Macaca
Title Vero-24h-S-2-Input
BioProject PRJCA003000
BioSample SAMC199629
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from tissues using TRIzol reagent (Invitrogen,15596018), followed by DNase I (NEB, M0303L) treatment to remove DNA contamination. Additional phenol-chloroform isolation and ethanol precipitation treatment was performed to remove enzyme contamination. total RNA was fragmented into ~130-nucleotide-long fragments by magnesium RNA fragmentation buffer (NEB, E6150S). The fragmentation was stopped by adding RNA fragmentation stop solution followed by ethanol precipitation. 8ng of fragmented total RNA was used as input and remained RNA was used to do the m6A-seq. The libraries were sequenced on Illumina Hiseq X10 with paired-end 2X 150 bp read length. RNA-Seq TRANSCRIPTOMIC PCR SINGLE
Processing Planned read length (bp): 150
Release date2021-02-22
Run
Run accession Run data file information
File nameFile size (MB)
CRR150991 CRR150991.fastq.gz 4,121.66
Submitterkai li (li_kai@pku.edu.cn)
OrganizationPeking University
Date submitted2020-07-13