Experiment information
Accession CRX122631
Organism human gut metagenome
Title Obesity-F-26
BioProject PRJCA002952
BioSample SAMC196517
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
DNA was extracted from the fecal samples using TIANamp Stool DNA kit according to the manufacturer’s instructions. The V4 region of the bacterial 16S rDNA was amplified by PCR using specific primers 515F and 806R. Sequencing libraries were generated using a NEBNext® Ultra™ DNA Library Prep kit from Illumina® and then sequenced on an Illumina Hiseq2590 platform. AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 400
Release date2020-07-06
Run
Run accession Run data file information
File nameFile size (MB)
CRR148738 CRR148738_f1.fq.gz
CRR148738_r2.fq.gz
4.98
5.44
SubmitterXiaowei Chen (chenxiaowei.cxw@qq.com)
OrganizationSoutheast University
Date submitted2020-07-03