Accession | CRX122627 |
Organism | human gut metagenome |
Title | Obesity-F-22 |
BioProject | PRJCA002952 |
BioSample | SAMC196513 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA was extracted from the fecal samples using TIANamp Stool DNA kit according to the manufacturer’s instructions. The V4 region of the bacterial 16S rDNA was amplified by PCR using specific primers 515F and 806R. Sequencing libraries were generated using a NEBNext® Ultra™ DNA Library Prep kit from Illumina® and then sequenced on an Illumina Hiseq2586 platform. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 400
|
Release date | 2020-07-06 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR148734 |
CRR148734_f1.fq.gz
CRR148734_r2.fq.gz
|
5.21
5.58
|
|
Submitter | Xiaowei Chen (chenxiaowei.cxw@qq.com) |
Organization | Southeast University |
Date submitted | 2020-07-03 |