| Accession | CRX121096 |
| Organism | Arabidopsis thaliana |
| Accession in Other Database |
GEO: GSM2096909 |
| Title | WT-INPUT-biological rep1 |
| BioProject | PRJCA002886 |
| BioSample | SAMC250389 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted with modified CTAB method. Total RNA was treated with RNase-free DNase I (New England BioLab) in order to remove DNA contamination for reverse transcription, or directly used for mRNA isolation. Poly-A RNA was isolated from total RNA using the Dynabeads Oligo dT (Invitrogen). The mRNA was quantified by Agilent 2100 Bioanalyzer. RNA was randomly fragmented to 100 nt by RNA Fragmentation Reagents (New England Biolab), and incubated with 5 ug m6A-specific antibody (Synaptic Systems) overnight at 4 degreeC. The m6A-containing fragments were pulled down with BSA pre-blocked Dynabeads Protein A (invitrogen), and eluted with 6.7mM m6A-containing buffer. Eluted RNA was precipitated by 75% ethanol. NEBNext Ultra RNA Library Prep Kit (E7530) was used for library building on input and IP sample. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 150
|
| Release date | 2020-10-11 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR147201 |
CRR147201_f1.fq.gz
CRR147201_r2.fq.gz
|
1,249.41
1,296.2
|
|
| Submitter | Hong-Chao Duan (duanhc@pku.edu.cn) |
|---|
| Organization | Peking University |
|---|
| Date submitted | 2020-06-20 |
|---|
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