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Experiment information
Accession CRX119199
Organism Sorghum bicolor
Title BS-seq
BioProject PRJCA002844
BioSample SAMC193431
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
1 μg of genomic DNA spiked with 1 ng unmethylated Lambda DNA was fragmented by sonication to a mean size of approximately 200-500 bp, then end-repaired, 5'-phosphorylated, 3'-dA-tailed and ligated to 5-methylcytosine modified adapters. After bisulfite treatment, the DNA was amplified with 10 cycles of PCR using Illumina 8 bp dual index primers. The constructed WGBS libraries were then analyzed by Agilent 2100 Bioanalyzer and finally sequenced on Illumina platforms using a 150×2 paired-end sequencing protocol. Bisulfite-Seq GENOMIC RANDOM PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2020-07-10
Run
Run accession Run data file information
File nameFile size (MB)
CRR145305 CRR145305_f1.fastq.gz
CRR145305_r2.fastq.gz 		5,756.95
5,890.33
SubmitterChao Zhou (zhouchao@ctgu.edu.cn)
OrganizationChina Three Gorges University
Date submitted2020-06-15
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