| Accession | CRX119195 |
| Organism | Sorghum bicolor |
| Title | Input_rep4 |
| BioProject | PRJCA002844 |
| BioSample | SAMC193427 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
5 μg of genomic DNA was fragmented by sonication to a mean size of approximately 200-400 bp, followed by end repair, A-base tailing and adapter ligation using the Acegen DNA Library Prep Kit (Acegen, Cat. No. AG0810) according to the manufacturer’s protocol. The adaptor-ligated DNA was immunoprecipitated by the anti-6mA antibody (Synaptic Systems, catalog number 202011). The captured DNA was then purified and amplified by 14-17 cycles of PCR using Illumina 8 bp dual index primers. Simultaneously, the input library was obtained from 5-10 ng of the ligated DNA before immunoprecipitation. The constructed libraries were then analyzed by Agilent 2100 Bioanalyzer and finally sequenced on Illumina platforms using a 150×2 paired-end sequencing protocol. |
MeDIP-Seq |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2020-07-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR145301 |
CRR145301_f1.fastq.gz
CRR145301_r2.fastq.gz
|
2,481.91
2,805.77
|
|
| Submitter | Chao Zhou (zhouchao@ctgu.edu.cn) |
|---|
| Organization | China Three Gorges University |
|---|
| Date submitted | 2020-06-15 |
|---|
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