Accession | CRX119187 |
Organism | Sorghum bicolor |
Accession in Other Database |
: |
Title | ATAC-seq_rep4 |
BioProject | PRJCA002844 |
BioSample | SAMC193419 |
Platform | Illumina NovaSeq 6000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The Nextera DNA Library Preparation Kit (Illumina) was used to perform the transposition according to the manufacturer’s manual. 50, 000 nuclei were pelleted and re-suspended with transposase for 30 minutes at 37 °C. The transposed DNA fragments were purified immediately using a MinElute PCR Purification Kit (Qiagen). After that, samples were PCR-amplified using 1 x NEBNext High-Fidelity PCR Master Mix (New England Biolabs, MA). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen) and subjected to sequencing on Illumina Novaseq 6000 using PE150. |
ATAC-seq |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2020-07-10 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR145293 |
CRR145293_f1.fastq.gz
CRR145293_r2.fastq.gz
|
4,136.39
3,801.5
|
|
Submitter | Chao Zhou (zhouchao@ctgu.edu.cn) |
Organization | China Three Gorges University |
Date submitted | 2020-06-15 |