| Accession | CRX119186 |
| Organism | Sorghum bicolor |
| Accession in Other Database |
: |
| Title | ATAC-seq_rep3 |
| BioProject | PRJCA002844 |
| BioSample | SAMC193418 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The Nextera DNA Library Preparation Kit (Illumina) was used to perform the transposition according to the manufacturer’s manual. 50, 000 nuclei were pelleted and re-suspended with transposase for 30 minutes at 37 °C. The transposed DNA fragments were purified immediately using a MinElute PCR Purification Kit (Qiagen). After that, samples were PCR-amplified using 1 x NEBNext High-Fidelity PCR Master Mix (New England Biolabs, MA). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen) and subjected to sequencing on Illumina Novaseq 6000 using PE150. |
ATAC-seq |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2020-07-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR145292 |
CRR145292_f1.fastq.gz
CRR145292_r2.fastq.gz
|
4,440.58
4,168.82
|
|
| Submitter | Chao Zhou (zhouchao@ctgu.edu.cn) |
|---|
| Organization | China Three Gorges University |
|---|
| Date submitted | 2020-06-15 |
|---|
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