Experiment information
Accession CRX119182
Organism Mus musculus
Title Hi-C_E6.5VE_rep2
BioProject PRJCA002851
BioSample SAMC193408
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
C57BL/6N female mice were induced to superovulation by injections of 5 IU of pregnant mare’s serum gonadotrophin(Ningbo Sansheng Pharmaceutical Co, Ltd) and 5 IU of human chorionic gonadotrophin(Ningbo Sansheng Pharmaceutical Co, Ltd) 44-48 hours apart and mated to PWK males. Embryos were flushed out from uterus at E4.5 and cultured 4 days in CMRL 1066 supplemented with 1mM glutamine, 1mM sodium pyruvate and 20% fetal bovine serum. After trypsin and pancreatic enzyme digestion followed by mechanical dissection to separate epiblast and visceral endoderm, embryonic cells were washed with PBS and incubated in lysis buffer.Hi-C libraries were prepared using Hi-C protocal developed by Rao et al. with slightly modification. Hi-C GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Planned read length (bp): 100
Release date2020-09-12
Run
Run accession Run data file information
File nameFile size (MB)
CRR145288 CRR145288_f1.fastq.gz
CRR145288_r2.fastq.gz
5,390.29
5,617.3
SubmitterYu Zhang (yuzhang0327@126.com)
OrganizationTsinghua University
Date submitted2020-06-15