| Accession | CRX119181 |
| Organism | Mus musculus |
| Title | Hi-C_E6.5VE_rep1 |
| BioProject | PRJCA002851 |
| BioSample | SAMC193407 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
C57BL/6N female mice were induced to superovulation by injections of 5 IU of pregnant mare’s serum gonadotrophin(Ningbo Sansheng Pharmaceutical Co, Ltd) and 5 IU of human chorionic gonadotrophin(Ningbo Sansheng Pharmaceutical Co, Ltd) 44-48 hours apart and mated to PWK males. Embryos were flushed out from uterus at E4.5 and cultured 4 days in CMRL 1066 supplemented with 1mM glutamine, 1mM sodium pyruvate and 20% fetal bovine serum. After trypsin and pancreatic enzyme digestion followed by mechanical dissection to separate epiblast and visceral endoderm, embryonic cells were washed with PBS and incubated in lysis buffer.Hi-C libraries were prepared using Hi-C protocal developed by Rao et al. with slightly modification. |
Hi-C |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Planned read length (bp): 100
|
| Release date | 2020-09-12 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR145287 |
CRR145287_f1.fastq.gz
CRR145287_r2.fastq.gz
|
4,333.66
4,419.76
|
|
| Submitter | Yu Zhang (yuzhang0327@126.com) |
|---|
| Organization | Tsinghua University |
|---|
| Date submitted | 2020-06-15 |
|---|
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