Experiment information
Accession CRX119177
Organism Mus musculus
Title BS-TELP mESC (100ng) rep1
BioProject PRJCA002851
BioSample SAMC193403
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Embryos were collected from C57BL/6N mice induced to superovulation by injections of 5 IU of pregnant mare’s serum gonadotrophin(Ningbo Sansheng Pharmaceutical Co, Ltd) and 5 IU of human chorionic gonadotrophin(Ningbo Sansheng Pharmaceutical Co, Ltd) 44-48 hours apart and mated to DBA/2N males. Embryos were washed with PBS and incubated in lysis buffer.BS-TELP-methylC libraries were prepared using TELP developed by PengXu et al. with slightly modification. Bisulfite-Seq GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Planned read length (bp): 100
Release date2020-09-12
Run
Run accession Run data file information
File nameFile size (MB)
CRR145283 CRR145283_f1.fastq.gz
CRR145283_r2.fastq.gz
7,603.9
7,435.7
SubmitterYu Zhang (yuzhang0327@126.com)
OrganizationTsinghua University
Date submitted2020-06-15