Accession | CRX119176 |
Organism | Mus musculus |
Title | mES 500cells R1 cell line BS_TELP |
BioProject | PRJCA002851 |
BioSample | SAMC193402 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Embryos were collected from C57BL/6N mice induced to superovulation by injections of 5 IU of pregnant mare’s serum gonadotrophin(Ningbo Sansheng Pharmaceutical Co, Ltd) and 5 IU of human chorionic gonadotrophin(Ningbo Sansheng Pharmaceutical Co, Ltd) 44-48 hours apart and mated to DBA/2N males. Embryos were washed with PBS and incubated in lysis buffer.BS-TELP-methylC libraries were prepared using TELP developed by PengXu et al. with slightly modification. |
Bisulfite-Seq |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Planned read length (bp): 100
|
Release date | 2020-09-12 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR145282 |
CRR145282_f1.fastq.gz
CRR145282_r2.fastq.gz
|
2,392.28
2,384.26
|
|
Submitter | Yu Zhang (yuzhang0327@126.com) |
Organization | Tsinghua University |
Date submitted | 2020-06-15 |