Experiment information
Accession CRX113975
Organism Mus musculus
Title adNIC2
BioProject PRJCA002745
BioSample SAMC187950
Platform Illumina HiSeq 3000
Library
Library name Construction protocol Strategy Source Selection Layout
vSMCs-DA transfected with AdNIC or AdCtrl for 48 h were subjected to RNA extraction, using the Trizol reagent (Thermo Fisher). The RNA integrity was evaluated using the Agilent 2200 Tape Station (Agilent Technologies, Santa Clara, CA, USA). Ribosomal RNA (rRNA) was removed using the Epicentre Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA, USA). The remaining RNA was fragmented into approximately 200 bp fragments. Subsequently, samples were subjected to first- and second-strand cDNA synthesis, followed by adaptor ligation and enrichment with a low-cycle PCR using Tru Seq® RNA LT/HT Sample Prep Kit (Illumina). The purified library products were evaluated using the Agilent 2200 Tape Station and Qubit® 2.0 (Life Technologies, Foster City, CA, USA) and then diluted to 10 pM for cluster generation in situ on the HiSeq3000 pair-end flow cell, followed by sequencing (2 × 150 bp) on HiSeq3000. RNA-Seq TRANSCRIPTOMIC RT-PCR PAIRED
Processing Planned read length (bp) for mate 1: 200
Planned read length (bp) for mate 2: 200
Release date2020-06-06
Run
Run accession Run data file information
File nameFile size (MB)
CRR139820 CRR139820_f1.fastq.gz
CRR139820_r2.fastq.gz
3,560.87
4,094.72
SubmitterXianchun Yan (yanxianchun@163.com)
OrganizationThe Fourth Military Medical University
Date submitted2020-05-29
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