Experiment information
Accession CRX113825
Organism Mus musculus
Title RNA-seq of mouse brain I1
BioProject PRJCA002742
BioSample SAMC187866
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
A total amount of 3 g RNA of ischemic area in MCAO mouse brain was used as input material for rRNA removal using Epicentre Ribo-Zero Gold Kit (Epicentre, USA). Library construction and sequencing were performed by Annoroad Gene Technology (Beijing, China). Sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturers recommendations and index codes were added to attribute sequences to each sample. RNA-Seq TRANSCRIPTOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Release date2020-06-01
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR139409 2020-06-01 I1_R1.fq.gz
I1_R2.fq.gz
3,730.3
4,001.35
SubmitterZelin Liu (zlliu@bjmu.edu.cn)
OrganizationsPeking University Health Science Center
Date submitted2020-05-27
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