Accession | CRX113825 |
Organism | Mus musculus |
Title | RNA-seq of mouse brain I1 |
BioProject | PRJCA002742 |
BioSample | SAMC187866 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A total amount of 3 g RNA of ischemic area in MCAO mouse brain was used as input material for rRNA removal using Epicentre Ribo-Zero Gold Kit (Epicentre, USA). Library construction and sequencing were performed by Annoroad Gene Technology (Beijing, China). Sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturers recommendations and index codes were added to attribute sequences to each sample. |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
Release date | 2020-06-01 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR139409 |
CRR139409_f1.fq.gz
CRR139409_r2.fq.gz
|
3,730.3
4,001.35
|
|
Submitter | Zelin Liu (zlliu@bjmu.edu.cn) |
Organization | Peking University Health Science Center |
Date submitted | 2020-05-27 |