Experiment information
Accession CRX113335
Organism Dendrobium officinale
Title RNA-Seq of CK2
BioProject PRJCA002680
BioSample SAMC185839
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNASeq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). RNA-Seq TRANSCRIPTOMIC unspecified PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 300
Release date2020-05-26
Run
Run accession Run data file information
File nameFile size (MB)
CRR138913 CRR138913_f1.fq.gz
CRR138913_r2.fq.gz
1,162.83
1,212.64
SubmitterXinqiao Zhan (qiao1605@126.com)
OrganizationZhejiang University
Date submitted2020-05-26
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