Experiment information
Accession CRX104779
Organism Bos taurus
Title T3_3A
BioProject PRJCA002228
BioSample SAMC173306
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
To comprehensively expound the adaptive changes of liver during transition period, liver biopsies were conducted twice on 20 transition cows at average 7d before calving and average 16d after calving. Transcriptome profile of the liver samples were detected using RNAseq. Accurate 3 μg RNA per sample was utilized as input for complementary DNA (cDNA) libraries construction. Sequencing libraries was generated using the rRNA-depleted RNA following manufacturer’s recommendations, with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA). The RNA sequencing was performed on an Illumina HiSeq 4000 platform at the Novogene Bioinformatics Institute (Beijing, China) according to the manufacturer’s instructions. RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2020-04-24
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR129369 2020-04-24 T3_3A_1.fq.gz
T3_3A_2.fq.gz
3,564.66
3,879.99
Submittergao shengtao (gaoshengtao1990@163.com)
OrganizationsInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences
Date submitted2020-04-20