Experiment information
Accession CRX104488
Organism Tupaia chinensis
Title PTH1-3
BioProject PRJCA002538
BioSample SAMC159791
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
A total of 2 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L; New England Biolabs (NEB), Ipswich, MA, USA) following the manufacturer’s recommendations, and index codes were given to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). First-strand cDNA was synthesized using random hexamer primer and RNase H. Second-strand cDNA synthesis was subsequently performed using buffer, dNTPs, DNA polymerase I, and RNase H. The library fragments were purified with QiaQuick PCR Kits (ID28104; QIAGEN, Germany) and eluted with EB buffer, after which terminal repair, A-tailing, and adapter addition were implemented. Then, the products were retrieved and amplified, and the library was completed. The libraries were sequenced on an Illumina platform, and 167 base-pair paired-end reads were generated. RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
Release date2020-04-19
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR129071 2020-04-19 PTH1-3_R1.fq.gz
PTH1-3_R2.fq.gz
1,992.67
2,086.91
SubmitterMaosen Ye (yemaosen@mail.kiz.ac.cn)
OrganizationsKunming Institute of Zoology, Chinese Academy of Sciences
Date submitted2020-04-16