Experiment information
Accession CRX104254
Organism Oryza meyeriana var. granulata
Title RNA sequencing of Oryza granulata (SL)
BioProject PRJCA001328
BioSample SAMC155195
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
A total of seven tissues representing different developmental stages of Oryza granulate were sampled to generate the RNA-Seq data needed for subsequent genome annotation. These tissues included panicles at three different stages of flower development, flag leaves, and stems and the shoots and roots of three-leaf seedlings. Because of the low germination rate of O. granulata, seedlings were germinated from seeds harvested from multiple plants, while the remaining tissues were sampled from the individual used for genome sequencing. All collected samples were quickly frozen in liquid nitrogen. RNA was individually extracted from each tissue using TRI reagent according to the instructions provided with the reagents. WGS TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 126
Planned read length (bp) for mate 2: 126
Insert size (bp): 180
Release date2020-04-13
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR128816 2020-04-13 Plant_S61-T04-I_good_1.fq.gz
Plant_S61-T04-I_good_2.fq.gz
1,900.42
1,972.54
SubmitterWei Li (liweisdau@126.com)
OrganizationsSouth China Agricultural University
Date submitted2020-04-13
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