Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
3-6kb |
Total RNAs with an RNA integrity number (RIN) value larger than 8.5 were mixed equally from 4 different tissues including root, stem, leaf and flower. Next, the cDNA synthesis was finished by The Clontech SMARTer PCR cDNA Synthesis Kit, where the reverse transcription (RT) was primed with an anchored oligo (dT) 30 primer. The product was separated by a BluePippin size selection system into 1-2kb, 2-3kb and over 3kb. The three SMRT libraries were constructed with Pacific Biosciences SMRTbell TEMPLATE Prep kit 1.0 according to the standard protocol. |
RNA-Seq |
TRANSCRIPTOMIC |
unspecified |
SINGLE
|
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