Experiment information
Accession CRX104169
Organism Panax notoginseng
Title Full-length mRNA sequencing of Panax notoginseng (E3)
BioProject PRJCA002506
BioSample SAMC154541
Platform PacBio RS II
Library
Library name Construction protocol Strategy Source Selection Layout
3-6kb Total RNAs with an RNA integrity number (RIN) value larger than 8.5 were mixed equally from 4 different tissues including root, stem, leaf and flower. Next, the cDNA synthesis was finished by The Clontech SMARTer PCR cDNA Synthesis Kit, where the reverse transcription (RT) was primed with an anchored oligo (dT) 30 primer. The product was separated by a BluePippin size selection system into 1-2kb, 2-3kb and over 3kb. The three SMRT libraries were constructed with Pacific Biosciences SMRTbell TEMPLATE Prep kit 1.0 according to the standard protocol. RNA-Seq TRANSCRIPTOMIC unspecified SINGLE
Processing
Release date2020-04-12
Run
Run accession Run data file information
File nameFile size (MB)
CRR128729 CRR128729.fastq.gz 73.95
CRR128730 CRR128730.fastq.gz 64.4
CRR128731 CRR128731.fastq.gz 69.34
SubmitterWei Li (liweisdau@126.com)
OrganizationSouth China Agricultural University
Date submitted2020-04-10
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