Accession | CRX103141 |
Organism | Pyrus pyrifolia |
Title | GR-9 |
BioProject | PRJCA002489 |
BioSample | SAMC153587 |
Platform | Illumina HiSeq 2000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes) or by removing rRNAs from the total RNA (for prokaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using Illumina HiSeq™ 2000. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
SINGLE
|
|
Processing |
Planned read length (bp): 150
|
Release date | 2020-08-17 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR127668 |
CRR127668.fq.gz
|
306.07
|
|
Submitter | Pujuan Zhang (keerhai2006@163.com) |
Organization | Nanjing Agricultural University, |
Date submitted | 2020-04-05 |