Experiment information
Accession CRX103141
Organism Pyrus pyrifolia
Title GR-9
BioProject PRJCA002489
BioSample SAMC153587
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes) or by removing rRNAs from the total RNA (for prokaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using Illumina HiSeq™ 2000. RNA-Seq TRANSCRIPTOMIC PCR SINGLE
Processing Planned read length (bp): 150
Release date2020-08-17
Run
Run accession Run data file information
File nameFile size (MB)
CRR127668 CRR127668.fq.gz 306.07
SubmitterPujuan Zhang (keerhai2006@163.com)
OrganizationNanjing Agricultural University,
Date submitted2020-04-05
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