Accession | CRX101347 |
Organism | Epinephelus fuscoguttatus |
Title | whole genome sequencing of brown-marbled grouper |
BioProject | PRJCA002402 |
BioSample | SAMC149141 |
Platform | OXFORD_NANOPORE GridION |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The large DNA fragments (10-50 Kb) were excised from the gel with Blue Pippin Automated DNA Size Selection System (Sage Science, Inc., MA, USA). The ends of DNA fragments were repaired and add A base with NEBNext Ultra End Repair/dA-Tailing Module (NEB, USA). The DNA fragments were ligated with adapters using Ligation Sequencing 1D Kit LSK109 (ONT, UK) following instruction. The prepared DNA were sequenced on FlowCells using the GridION ×5 sequencer (ONT). |
WGA |
GENOMIC |
unspecified |
SINGLE
|
|
Processing |
Planned read length (bp): 10000
|
Release date | 2020-04-19 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR125451 |
CRR125451.fastq.gz
|
9,383.37
|
CRR125452 |
CRR125452.fastq.gz
|
54,304.13
|
CRR125453 |
CRR125453.fastq.gz
|
23,539.17
|
|
Submitter | yang yang (214453598@qq.com) |
Organization | Sun Yat-sen University |
Date submitted | 2020-03-18 |