Experiment information
Accession CRX101347
Organism Epinephelus fuscoguttatus
Title whole genome sequencing of brown-marbled grouper
BioProject PRJCA002402
BioSample SAMC149141
Platform OXFORD_NANOPORE GridION
Library
Library name Construction protocol Strategy Source Selection Layout
The large DNA fragments (10-50 Kb) were excised from the gel with Blue Pippin Automated DNA Size Selection System (Sage Science, Inc., MA, USA). The ends of DNA fragments were repaired and add A base with NEBNext Ultra End Repair/dA-Tailing Module (NEB, USA). The DNA fragments were ligated with adapters using Ligation Sequencing 1D Kit LSK109 (ONT, UK) following instruction. The prepared DNA were sequenced on FlowCells using the GridION ×5 sequencer (ONT). WGA GENOMIC unspecified SINGLE
Processing Planned read length (bp): 10000
Release date2020-04-19
Run
Run accession Run data file information
File nameFile size (MB)
CRR125451 CRR125451.fastq.gz 9,383.37
CRR125452 CRR125452.fastq.gz 54,304.13
CRR125453 CRR125453.fastq.gz 23,539.17
Submitteryang yang (214453598@qq.com)
OrganizationSun Yat-sen University
Date submitted2020-03-18
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