Experiment information
Accession CRX094065
Organism Nicotiana tabacum
Title Tobacco_WT_3hpi_7
BioProject PRJCA002284
BioSample SAMC138109
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
The normal tobacco was treated with gray mold, and samples were collected at 0hpi, 0.5hpi and 3hpi after inoculation for transcriptome sequencing analysis, and the sampled data followed 3 biological replications. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq Xten platform and paired-end reads were generated. RNA-Seq TRANSCRIPTOMIC unspecified PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 240
Release date2020-02-29
Run
Run accession Run data file information
File nameFile size (MB)
CRR118784 CRR118784_f1.fq.gz
CRR118784_r2.fq.gz
3,812.23
4,206.26
SubmitterChao Gong (gongchao@gdaas.cn)
Organization Guangdong Academy of Agricultural Sciences
Date submitted2020-02-28