| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The nCov infected whole-BALF sample was used to extract RNA using Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA) and Trizol LS (Thermo Fisher Scientific, Carlsbad, CA, USA) in biosafety III laboratory The RNA was then reverse transcribed, and amplified using an Ovation Trio RNA-Seq library preparation kit (NuGEN, CA, USA) and was sequenced on an Illumina HiSeq 2500/4006 platform (Illumina, United Kingdom),so it's a single and pair end merged bam |
RNA-Seq |
METATRANSCRIPTOMIC |
RT-PCR |
SINGLE
|
|
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