Experiment information
Accession CRX091748
Organism Macaca fascicularis
Title cmESC1
BioProject PRJCA002195
BioSample SAMC134274
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
The cmESCs were cultured in PM, FM, and DM. Porcine PA blastocysts were collected 5 day after activation and cultured in PM, FM, and DM for 48 h. The cmESCs and porcine PA blastocysts were then collected for RNA-seq analysis. Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, 15596026). For RNA-seq library construction, PolyA+ tailed RNA purification was performed for each sample using an E.Z.N.A. MicroElute RNA Clean-Up Kit (Omega Bio-tek). Sequencing was performed on an Illumina HiSeq 4000 sequencer with 150-bp paired-end sequencing reactions. RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2020-02-08
Run
Run accession Run data file information
File nameFile size (MB)
CRR116138 CRR116138_f1.fq.gz
CRR116138_r2.fq.gz
1,530.39
1,816.46
SubmitterGuihai Feng (fenggh@aliyun.com)
OrganizationInstitute of Zoology, Chinese Academy of Sciences
Date submitted2020-02-08
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