Accession | CRX091748 |
Organism | Macaca fascicularis |
Title | cmESC1 |
BioProject | PRJCA002195 |
BioSample | SAMC134274 |
Platform | Illumina HiSeq 4000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The cmESCs were cultured in PM, FM, and DM. Porcine PA blastocysts were collected 5 day after activation and cultured in PM, FM, and DM for 48 h. The cmESCs and porcine PA blastocysts were then collected for RNA-seq analysis. Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, 15596026). For RNA-seq library construction, PolyA+ tailed RNA purification was performed for each sample using an E.Z.N.A. MicroElute RNA Clean-Up Kit (Omega Bio-tek). Sequencing was performed on an Illumina HiSeq 4000 sequencer with 150-bp paired-end sequencing reactions. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2020-02-08 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR116138 |
CRR116138_f1.fq.gz
CRR116138_r2.fq.gz
|
1,530.39
1,816.46
|
|
Submitter | Guihai Feng (fenggh@aliyun.com) |
Organization | Institute of Zoology, Chinese Academy of Sciences |
Date submitted | 2020-02-08 |