Experiment information
Accession CRX091308
Organism Bos taurus
Title siNCAPG2
BioProject PRJCA002153
BioSample SAMC133169
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
The ATAC-seq libraries were performed as TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, Nanjing, China). Briefly, collected 50,000 cells (counted by using trypan blue exclusion) were lysed in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40, 0.1% Tween-20 and 0.01% Digitalis saponin) for 10 min on ice. After a centrifugation at 500g for 5 min, the nuclei were pelleted and resuspended in Transposase buffer. The transposition reaction was carried out for 30 min at 37°C. Following purification, the libraries were amplified for 16 cycles and purified using the VAHTS RNA Clean Beads (Vazyme, Nanjing, China). ATAC-seq GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 150
Release date2020-01-24
Run
Run accession Run data file information
File nameFile size (MB)
CRR115699 CRR115699_f1.fq.gz
CRR115699_r2.fq.gz
3,692.21
3,985.5
SubmitterLupei Zhang (zhanglupei@caas.cn)
OrganizationInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences
Date submitted2020-01-24
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