Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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The ATAC-seq libraries were performed as TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, Nanjing, China). Briefly, collected 50,000 cells (counted by using trypan blue exclusion) were lysed in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40, 0.1% Tween-20 and 0.01% Digitalis saponin) for 10 min on ice. After a centrifugation at 500g for 5 min, the nuclei were pelleted and resuspended in Transposase buffer. The transposition reaction was carried out for 30 min at 37°C. Following purification, the libraries were amplified for 16 cycles and purified using the VAHTS RNA Clean Beads (Vazyme, Nanjing, China). |
ATAC-seq |
GENOMIC |
PCR |
PAIRED
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