Experiment information
Accession CRX082713
Organism Mus musculus
Title puromycin
BioProject PRJCA002101
BioSample SAMC127078
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Inserted sites were isolated using splinkerette PCR and high throughput sequencing. The second round of the nested PCR primers was used as barcodes to filter reads. We then extracted the sequences contained "TTAA" using cutadapt, and then aligned them to mouse reference genome from ensembl release 97 using bwa aligner. PCR duplications in alignments were removed using gatk, and cleaned alignments were used to count reads within the reference gtf AMPLICON GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Release date2020-01-08
Run
Run accession Run data file information
File nameFile size (MB)
CRR106360 CRR106360_f1.fastq.gz
CRR106360_r2.fastq.gz
1,730.37
1,963.12
SubmitterJunjie Mao (learnbetter@hotmail.com)
OrganizationInstitute of Zoology, Chinese Academy of Sciences
Date submitted2020-01-07
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