Accession | CRX079864 |
Organism | Mus musculus |
Title | Nt |
BioProject | PRJCA002022 |
BioSample | SAMC124188 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Single-cell suspensions with 1×10^5 cells/mL in concentration in PBS (HyClone) were prepared. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPETM protocol by GEXSCOPETM Single-Cell RNA Library Kit (Singleron Biotechnologies) . Individual libraries were diluted to 4nM and pooled for sequencing. Pools were sequenced on Illumina HiSeq X with 150 bp paired end reads. |
RNA-Seq |
METATRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
Release date | 2019-12-19 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR102636 |
CRR102636_f1.fq.gz
CRR102636_r2.fq.gz
|
25,961.61
27,290.55
|
|
Submitter | Yinchuan Li (18622397604@163.com) |
Organization | Nantong University |
Date submitted | 2019-12-17 |