Experiment information
Accession CRX079864
Organism Mus musculus
Title Nt
BioProject PRJCA002022
BioSample SAMC124188
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Single-cell suspensions with 1×10^5 cells/mL in concentration in PBS (HyClone) were prepared. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPETM protocol by GEXSCOPETM Single-Cell RNA Library Kit (Singleron Biotechnologies) . Individual libraries were diluted to 4nM and pooled for sequencing. Pools were sequenced on Illumina HiSeq X with 150 bp paired end reads. RNA-Seq METATRANSCRIPTOMIC RT-PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2019-12-19
Run
Run accession Run data file information
File nameFile size (MB)
CRR102636 CRR102636_f1.fq.gz
CRR102636_r2.fq.gz
25,961.61
27,290.55
SubmitterYinchuan Li (18622397604@163.com)
OrganizationNantong University
Date submitted2019-12-17
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