Experiment information
Accession CRX078173
Organism Populus x canadensis
Title Populus x canadensis 1
BioProject PRJCA001984
BioSample SAMC122138
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA of samples was extracted by the CTAB method. RNA degradation and contamination was monitored on 1% agarose gels, and RNA purity was checked using the NanoPhotometer® spectrophotometer. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system. Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. At last, library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2500 platform and 50bp single-end reads were generated.The small RNA sequencing libraries were generated using RNase R digested and rRNA-depleted RNAs by NEBNext Ultra Directional RNA Library Prep Kit for Illumina following manufacturer’s recommendations. After cluster generation on a cBot Cluster Generation System using HiSeq PE Cluster Kit v4 cBot or TruSeq SR Cluster Kit v3-cBot-HS (Illumina), the library preparations were sequenced on an Illumina Hiseq 2500 platform. miRNA-Seq TRANSCRIPTOMIC RT-PCR FRAGMENT
Processing Planned read length (bp): 50
Release date2019-12-10
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR100914 2019-12-10 pcq1_raw.fq.gz 513.48
Submitterjiahong xu  (jiahongxuhn@sina.com)
OrganizationsHainan University
Date submitted2019-12-10
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