Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Transposition reactions were incubated at 37 °C for 30 min in an Eppendorf ThermoMixer with shaking at 1,000 r.p.m. Following purification, we amplified library fragments using PCR .Tagmented DNA was purified using the MinElute Reaction Cleanup Kit (Qiagen, 28204). After the library was amplified by PCR and subjected to paired-end 35 bp high-throughput sequencing on an Illumina NextSeq 500 Next Generation Sequencing platform. |
ATAC-seq |
GENOMIC |
other |
PAIRED
|
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