Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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For SMRTbell libraries, equal amounts of total RNA from biological replicates, which were used for RNA sequencing libraries, were combined to generate two RNA pools, the control pool and the heat stress pool. First, oligo was used to enrich for mRNA containing a poly-A tail, then mRNA was reverse transcribed into cDNA using the SMARTer PCR cDNA Synthesis Kit. The cDNA was amplified by PCR. The fragments were then screened for large-scale PCR to obtain sufficient cDNA. The full-length cDNA obtained was subjected to injury repair, end-repair, ligation of SMRT dumbbell-type linkers, and construction of a full length transcriptome library. We removed the sequence of the unligated linker at both ends of the cDNA and then bound the primer and the binding DNA polymerase to form a complete SMRTbell library. |
FL-cDNA |
TRANSCRIPTOMIC |
RT-PCR |
SINGLE
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