Experiment information
Accession CRX075456
Organism Populus x canadensis
Title Populus x canadensis
BioProject PRJCA001975
BioSample SAMC117012
Platform PacBio Sequel
Library
Library name Construction protocol Strategy Source Selection Layout
For SMRTbell libraries, equal amounts of total RNA from biological replicates, which were used for RNA sequencing libraries, were combined to generate two RNA pools, the control pool and the heat stress pool. First, oligo was used to enrich for mRNA containing a poly-A tail, then mRNA was reverse transcribed into cDNA using the SMARTer PCR cDNA Synthesis Kit. The cDNA was amplified by PCR. The fragments were then screened for large-scale PCR to obtain sufficient cDNA. The full-length cDNA obtained was subjected to injury repair, end-repair, ligation of SMRT dumbbell-type linkers, and construction of a full length transcriptome library. We removed the sequence of the unligated linker at both ends of the cDNA and then bound the primer and the binding DNA polymerase to form a complete SMRTbell library. FL-cDNA TRANSCRIPTOMIC RT-PCR SINGLE
Processing
Release date2019-12-10
Run
Run accession Run data file information
File nameFile size (MB)
CRR088467 CRR088467.bam 15,726.46
Submitterjiahong xu  (jinhuichen@hainanu.edu.cn)
OrganizationHainan University
Date submitted2019-11-30
Related experiments
Experiments(0)