| Accession | CRX074570 |
| Organism | Gallus gallus |
| Title | FL_M1 |
| BioProject | PRJCA001898 |
| BioSample | SAMC116236 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A total amount of 5.2 microgram genomic DNA spiked with 26 ng lambda DNA were fragmented by sonication to 200-300bp with Covaris S220, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA as per manufacturer’s instructions.Then these DNA fragments
were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit
(Zymo Research), before the resulting single-strand DNA fragments were PCR
amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X) |
Bisulfite-Seq |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2019-11-23 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR087499 |
CRR087499_f1.fq.gz
CRR087499_r2.fq.gz
|
8,275.29
7,903.68
|
|
| Submitter | Xiaodong Tan (tanxiaodong08@163.com) |
|---|
| Organization | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
|---|
| Date submitted | 2019-11-22 |
|---|
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