Accession | CRX074559 |
Organism | Gallus gallus |
Title | C_R1 |
BioProject | PRJCA001898 |
BioSample | SAMC116225 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Firstly, ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNAdepleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations |
ncRNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2019-11-23 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR087488 |
CRR087488_f1.fq.gz
CRR087488_r2.fq.gz
|
3,877.9
4,582.49
|
|
Submitter | Xiaodong Tan (tanxiaodong08@163.com) |
Organization | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
Date submitted | 2019-11-22 |