Experiment information
Accession CRX072961
Organism Bacteria
Title N40-3
BioProject PRJCA001919
BioSample SAMC114557
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
The DNA of sludge samples were extracted. Primers 515F (5’-GTGCCAGCMGCCGCGGTAA-3’) and 818R (5’-GGACTACHVGGGTWTCTAAT-3’) were used for 16S rRNA gene V4 region amplification. The PCR products with different barcodes were purified, quantified, and pooled tighter to construct the DNA library, and high throughput sequencing was performed on Illumina sequencing platform. AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 219
Release date2019-11-18
Run
Run accession Run data file information
File nameFile size (MB)
CRR085738 CRR085738_f1.fq.gz
CRR085738_r2.fq.gz
7.07
6.37
SubmitterQiao Ma (xiaoma0556@qq.com)
OrganizationDalian Maritime University
Date submitted2019-11-17