Experiment information
Accession CRX072134
Organism freshwater metagenome
Title S14-1
BioProject PRJCA001909
BioSample SAMC114102
Platform Illumina HiSeq 3000
Library
Library name Construction protocol Strategy Source Selection Layout
Approximately 500 ml water of each sample were filtrated by filtrating membrane with 0.22 μm of aperture for collecting microorganisms. Then the filtrating membrane was cut into pieces and microbial DNA was extracted using a PowerSoil DNA isolation kit. DNA concentration and quality were checked using a NanoDrop spectrophotometer. The V4 hypervariable region of the prokaryotic 16S rRNA gene was amplified using the universal primer pair 515F and 909R. Polymerase chain reaction were performed and the amplicons were sequenced using a HiSeq system at Guangdong Meilikang Bio-Science. AMPLICON METAGENOMIC PCR SINGLE
Processing Planned read length (bp): 420
Release date2019-11-15
Run
Run accession Run data file information
File nameFile size (MB)
CRR084879 CRR084879.fastq.gz 5.14
SubmitterXiaojuan Chen (chenxiaojuan@mail.ihe.ac.cn)
OrganizationInstitute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences
Date submitted2019-11-14