Accession | CRX071771 |
Organism | Cuscuta australis |
Title | SGJ-LD-C4 |
BioProject | PRJCA001885 |
BioSample | SAMC113384 |
Platform | Illumina HiSeq 4000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was isolated from samples using the Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions. RNA quality and quantity were determined with a spectrophotometer (IMPLEN, Germany) and an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RNA-seq was performed at the Novogene Company (Beijing, China). The RNA-Seq library was constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The cleaved RNA fragments were transcribed into first-strand cDNA using reverse transcriptase and subsequently second-strand cDNA synthesis was performed using DNA polymerase I and RNase H. The fragments were ligated to sequencing adaptors and the library preparations were sequenced on an Illumina HiSeq 4000 platform and 150 bp paired-end reads were generated. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
Release date | 2019-11-06 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR084304 |
CRR084304_f1.fq.gz
CRR084304_r2.fq.gz
|
1,291.31
1,348.49
|
|
Submitter | Yuxing Xu (xuyuxing@mail.kib.ac.cn) |
Organization | Chinese Academy of Sciences |
Date submitted | 2019-11-06 |