Experiment information
Accession CRX065682
Organism Enterobacteriaceae bacterium
Title AF18-NC_1
BioProject PRJCA001829
BioSample SAMC111164
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
AF18 and AF18-NC cells were harvest from its overnight culture by centrifuge, and total RNA of both strains were extracted with QIAGEN RNeasy Plus Mini Kit (Qiagen, Cat No.74134) following manufacturer’s instruction. The extracted RNA samples were assayed with NanoDrop spectrophotometer for quantification and then sent to Beijing Novogene Co., LTD for transcriptome sequencing. After deletion of rRNA from samples, transcriptome was sequenced on Hiseq 2000 platform with an intended depth of 1G of raw 100bp×2 reads RNA-Seq TRANSCRIPTOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 200
Release date2020-02-29
Run
Run accession Run data file information
File nameFile size (MB)
CRR078103 CRR078103_f1.fq.gz
CRR078103_r2.fq.gz
383.61
437.75
Submittershi xing (shixing@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2019-10-14
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