Accession | CRX065682 |
Organism | Enterobacteriaceae bacterium |
Title | AF18-NC_1 |
BioProject | PRJCA001829 |
BioSample | SAMC111164 |
Platform | Illumina HiSeq 2000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
AF18 and AF18-NC cells were harvest from its overnight culture by centrifuge, and total RNA of both strains were extracted with QIAGEN RNeasy Plus Mini Kit (Qiagen, Cat No.74134) following manufacturer’s instruction. The extracted RNA samples were assayed with NanoDrop spectrophotometer for quantification and then sent to Beijing Novogene Co., LTD for transcriptome sequencing. After deletion of rRNA from samples, transcriptome was sequenced on Hiseq 2000 platform with an intended depth of 1G of raw 100bp×2 reads |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 200
|
Release date | 2020-02-29 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR078103 |
CRR078103_f1.fq.gz
CRR078103_r2.fq.gz
|
383.61
437.75
|
|
Submitter | shi xing (shixing@big.ac.cn) |
Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
Date submitted | 2019-10-14 |