Experiment information
Accession CRX062854
Organism Mus musculus
Title HFD_wt_RNA-seq_rep2
BioProject PRJCA001786
BioSample SAMC108813
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted with TRIzol reagent (Invitrogen, 15596-018) from the whole liver, mRNA was purified from total RNAs using Dynabeads mRNA purification kit (Ambion, 61006). RNA-seq libraries were directly generated using the KAPA Stranded Mrna-Seq Kit (KAPA, KK8401) following the manufacturer’s instructions, and then sequenced using the Illumina HiSeq 2000 platform. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
Release date2020-11-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR073691 CRR073691_f1.gz
CRR073691_r2.gz
5,190.08
5,968.48
Submittersun baofa (sunbf@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2019-09-27