Experiment information
Accession CRX062295
Organism Zea mays
Title R6-2
BioProject PRJCA001736
BioSample SAMC106515
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted with the RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA) according to the standard protocol, and mRNA was purified from total RNA using the Dynabeads mRNA Purification Kit (Invitrogen, USA). The polyA containing mRNA was purified with oligo-dT attached magnetic beads and RNA was fragmented and primed for cDNA synthesis during elution of the polyA RNA according to the standard protocol. The first stranded cDNA was synthesized using SuperScript II Reverse Transcriptase (Invitrogen, USA) and random primers (Invitrogen, USA), followed by second strand cDNA synthesis. The overhangs resulting from fragmentation were converted into blunt ends using an End Repair Mix (Invitrogen, USA). The 3\' to 5\' exonuclease activity of this mix removes the 3\' overhangs and the polymerase activity fills in the 5\' overhangs. After adenylation of 3 ends of DNA fragments, the Adaptors provided by Illumina were ligated. The fragments were amplified by PCR and 400-500 bp cDNA fragments were purified and sequenced with Illumina system Hiseq2000. Sequencing was performed by Beijing BerryGenomics Co., Ltd. (China) RNA-Seq TRANSCRIPTOMIC RANDOM PCR PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 300
Release date2020-06-30
Run
Run accession Run data file information
File nameFile size (MB)
CRR072971 CRR072971_f1.fastq.gz
CRR072971_r2.fastq.gz
2,389.69
2,373.08
SubmitterZhen Yan (yanzhensky@ibcas.ac.cn)
OrganizationInstitute of Botany, Chinese Academy of Sciences
Date submitted2019-09-11