Accession | CRX061281 |
Organism | Homo sapiens |
Title | RKO-ph7.4-1 |
BioProject | PRJCA001709 |
BioSample | SAMC097229 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The total RNA in RKO cells cultured in pH 7.4 and pH 6.8 conditions was isolated with TRIzol reagent(Life Technologies). The RNA integrity was measured at an Agilent Bioanalyzer 2100 instrument (Agilent Technologies, Santa Clara, CA,USA). The qualified total RNA was further purified (RNeasy Mini Kit, Qiagen, Hilden, Germany). |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
Release date | 2019-08-31 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR071910 |
CRR071910_f1.fq.gz
CRR071910_r2.fq.gz
|
3,574.48
3,763.07
|
|
Submitter | Peishan Hu (xiaopei296@sina.com) |
Organization | Sun Yat-sen University Cancer Center |
Date submitted | 2019-08-30 |