Experiment information
Accession CRX057166
Organism soil metagenome
Title NP1.05.2
BioProject PRJCA001644
BioSample SAMC088939
Platform Illumina MiSeq
Library name Construction protocol Strategy Source Selection Layout
Total bacterial genomic DNA samples were extracted using the Fast DNA SPIN extraction kits,and then PCR amplification of the bacterial 16S rRNA genes V3–V4 region was performed using the forward primer 338F and the reverse primer 806R. PCR amplicons were purified with Agencourt AMPure Beads and quantified using the PicoGreen dsDNA Assay Kit. Amplicons were pooled in equal amounts, and pair-end 2×300 bp sequencing was performed using the Illlumina MiSeq platform with MiSeq Reagent Kit v3. AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 500
Planned read length (bp) for mate 2: 500
Insert size (bp): 470
Release date2019-08-12
Run accession Release date Run data file information
File nameFile size (MB)
CRR063249 2019-08-12 D8l3500-50_R1.fq.gz
SubmitterDianming Wu (dmwu@geo.ecnu.edu.cn)
OrganizationsEast China Normal University
Date submitted2019-08-09