Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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Genomic DNA (1.5 μg per sample) was extracted from the liver tissue. Sequencing libraries were generated using a Truseq Nano DNA HT Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions. Index codes were added to identify sequences from each sample. Briefly, the DNA sample was fragmented by sonication to short-insert (180 bp and 500 bp) for paired-end and long-insert (2 kb and 5 kb) for mate-pair sequencing. Next, the DNA fragments were end-polished, A-tailed, and ligated with the full-length adapters for Illumina sequencing with further PCR amplification. Finally, PCR products were purified (AMPure XP system, Beckman Coulter, USA), and libraries were analyzed for size distribution using an Agilent 2100 Bioanalyzer, and quantified using real-time PCR. |
WGS |
GENOMIC |
RANDOM |
PAIRED
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