Experiment information
Accession CRX056739
Organism Artibeus jamaicensis
Title Ajam_250
BioProject PRJCA001630
BioSample SAMC088520
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Genomic DNA (1.5 μg per sample) was extracted from the liver tissue. Sequencing libraries were generated using a Truseq Nano DNA HT Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions. Index codes were added to identify sequences from each sample. Briefly, the DNA sample was fragmented by sonication to short-insert (180 bp and 500 bp) for paired-end and long-insert (2 kb and 5 kb) for mate-pair sequencing. Next, the DNA fragments were end-polished, A-tailed, and ligated with the full-length adapters for Illumina sequencing with further PCR amplification. Finally, PCR products were purified (AMPure XP system, Beckman Coulter, USA), and libraries were analyzed for size distribution using an Agilent 2100 Bioanalyzer, and quantified using real-time PCR. WGS GENOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 250
Release date2020-08-03
Run
Run accession Run data file information
File nameFile size (MB)
CRR062814 CRR062814_f1.fastq.gz
CRR062814_r2.fastq.gz
20,334
20,970.06
CRR062815 CRR062815_f1.fq.gz
CRR062815_r2.fq.gz
7,917.37
7,865.94
CRR062816 CRR062816_f1.fq.gz
CRR062816_r2.fq.gz
1,577.84
1,521.46
SubmitterKai Wang (kai_wang@whu.edu.cn)
OrganizationWuhan University
Date submitted2019-08-06
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