Experiment information
Accession CRX081442
Organism leaf metagenome
Title 16S amplicon sequencing of rice leaf microbiome sample V4leaf5
BioProject PRJCA001629
BioSample SAMC125247
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
DNA for each sample was extracted,then V4(515F-806R) of 16S rDNA was amplified by two-step PCR,and the amplicon was not digested using Cas9/mt-gRNA780 and cp-gRNA578.Finally,the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 300
Release date2020-01-04
Run accession Release date Run data file information
File nameFile size (MB)
CRR104402 2020-01-04 V4leaf5_S90_L001_R1_001.fastq.gz
SubmitterLu yang Song (lyang.s@webmail.hzau.edu.cn)
OrganizationsNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University
Date submitted2019-12-25