Experiment information
Accession CRX081440
Organism root metagenome
Title 16S amplicon sequencing of bulk soil microbiome sample V4leaf3
BioProject PRJCA001629
BioSample SAMC125245
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
DNA for each sample was extracted, then V4(515F-806R) of 16S rDNA was amplified by two-step PCR. Finally, the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 300
Release date2020-01-04
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR104400 2020-01-04 V4leaf3_S86_L001_R1_001.fastq.gz
V4leaf3_S86_L001_R2_001.fastq.gz
22.97
22.83
SubmitterLu yang Song (lyang.s@webmail.hzau.edu.cn)
OrganizationsNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University
Date submitted2019-12-25