Experiment information
Accession CRX056450
Organism root metagenome
Title 16S amplicon sequencing of rice root microbiome sample OsRE3-1
BioProject PRJCA001629
BioSample SAMC088218
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
DNA for each sample was extracted,then V5-V7(799F-1193R) of 16S rDNA was amplified by two-step PCR,and the amplicon was digested using Cas9/mt-gRNA1196,and second step PCR is 8 cycles.Finally,the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 394
Release date2020-01-04
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR062525 2020-01-04 OsRE3-1_S123_L001_R1_001.fastq.gz
OsRE3-1_S123_L001_R2_001.fastq.gz
22.35
27.28
SubmitterLu yang Song (lyang.s@webmail.hzau.edu.cn)
OrganizationsNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University
Date submitted2019-08-04