Accession | CRX053099 |
Organism | Lolium multiflorum |
Title | Italian ryegrass seedling iso-seq 3-10K |
BioProject | PRJCA001581 |
BioSample | SAMC073174 |
Platform | PacBio Sequel |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
3-10K |
2μg mRNA (mixed with three control and three treatment total RNAs) was used for cDNA library construction. Poly(A) RNAs were purified using the Poly(A) PuristTM Kit (Thermo Scientific). Poly(A) RNAs were reverse transcribed into cDNA using the SMARTer™ PCR cDNA Synthesis Kit (Clontech Laboratories). 3-10 kb, were separately collected from each mixed RNA by BluePippin Size Selection (Sage Science, Beverly, MA). Each library underwent SMRT sequencing using a SMRT cell on PacBio Sequel platform. The LD-PCR included 20 cycles. |
FL-cDNA |
TRANSCRIPTOMIC |
PolyA |
SINGLE
|
|
Processing |
|
Release date | 2020-02-13 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR059192 |
CRR059192.tar.gz
|
42,833.96
|
|
Submitter | Zhaoyang Hu (809263131@qq.com) |
Organization | Nanjing Agricultural University |
Date submitted | 2019-07-15 |