Experiment information
Accession CRX053099
Organism Lolium multiflorum
Title Italian ryegrass seedling iso-seq 3-10K
BioProject PRJCA001581
BioSample SAMC073174
Platform PacBio Sequel
Library
Library name Construction protocol Strategy Source Selection Layout
3-10K 2μg mRNA (mixed with three control and three treatment total RNAs) was used for cDNA library construction. Poly(A) RNAs were purified using the Poly(A) PuristTM Kit (Thermo Scientific). Poly(A) RNAs were reverse transcribed into cDNA using the SMARTer™ PCR cDNA Synthesis Kit (Clontech Laboratories). 3-10 kb, were separately collected from each mixed RNA by BluePippin Size Selection (Sage Science, Beverly, MA). Each library underwent SMRT sequencing using a SMRT cell on PacBio Sequel platform. The LD-PCR included 20 cycles. FL-cDNA TRANSCRIPTOMIC PolyA SINGLE
Processing
Release date2020-02-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR059192 CRR059192.tar.gz 42,833.96
SubmitterZhaoyang Hu (809263131@qq.com)
OrganizationNanjing Agricultural University
Date submitted2019-07-15
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