| Accession | CRX052866 |
| Organism | Mus musculus |
| Title | Rrm14 KO 3 |
| BioProject | PRJCA001576 |
| BioSample | SAMC073156 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| Rbm14 KO 3 |
RNA isolation and RNA-seq were performed as previously described. Briefly, 5 × 106 ES cells were harvested and lysed using 1 mL TRIzol reagent (Invitrogen). RNA was then purified with 1:5 (v/v) chloroform (Sinopharm Chemical Reagent Beijing Co., Ltd) and precipitated by centrifugation using 1:1 (v/v) isopropanol alcohol (Sinopharm Chemical Reagent Beijing Co., Ltd). The RNA pallet was then dissolved in RNase-free ddH2O (Invitrogen) at a concentration of about 1 μg/μL. cDNA libraries were constructed with the TruSeq RNA Sample Prep Kit using 10 μg of the isolated RNA per sample. All samples were sequenced by a Illumina HiSeq-X TEN sequencing system with paired-end 150-bp read length. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2019-10-25 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR058586 |
CRR058586_f1.fq.gz
CRR058586_r2.fq.gz
|
1,991.03
2,161.06
|
|
| Submitter | Guihai Feng (fenggh@aliyun.com) |
|---|
| Organization | Institute of Zoology, Chinese Academy of Sciences |
|---|
| Date submitted | 2019-07-12 |
|---|
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