| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
After the nucleic acid sample quality inspection was qualified, the genomic DNA was randomly interrupted by Megaruptor (Diagenode), the large fragment DNA was enriched and purified by magnetic beads. Both ends of the fragments were repaired and added dA tail. After purification, the SQK-LSK108 ligation kit (Oxford Nanopore Technologies) was used to perform the ligation reaction, and finally used Qubit to accurately quantify the constructed DNA library. The DNA library of the specified concentration and volume was added to a flow cell, and the flow cell was transferred to a PromethION sequencer for real-time single molecule sequencing. |
WGS |
METAGENOMIC |
unspecified |
SINGLE
|
|
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