Experiment information
Accession CRX052342
Organism Microbiota
Title 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R04
BioProject PRJCA001267
BioSample SAMC072577
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
DNA for each sample was extracted with FastDNA SPIN Kit (MP Biomedicals), then V5-V7(799F-1193R) of 16S rDNA was amplified by two-step PCR. Finally, the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode. AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 450
Release date2019-07-05
Run accession Release date Run data file information
File nameFile size (MB)
CRR058056 2019-07-05 ColPhyl2R04_1.fq.gz
SubmitterYong-Xin Liu (woodcorpse@163.com)
OrganizationsInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
Date submitted2019-07-05