Experiment information
Accession CRX052293
Organism Litchi chinensis
Title MM3D-2
BioProject PRJCA001541
BioSample SAMC072543
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted using RNAprep Pure Plant Kit (Polysaccharides & Polyphenolics-rich) (Tiangen Biotech, China). For RNA-seq library construction, 5?g of total RNA with appropriate amount of Oligo-dT25 beads (Invitrogen, USA) was used. The mRNA was fragmented into short fragments, and reversed transcribed into cDNA by random primers. Second-strand cDNA was synthesized using DNA polymerase I, RNase H, dNTPs. After that, the cDNA fragments were purified by Agencourt AMPure XP (Beckman Coulter, USA). The purified cDNA fragments were end repaired, added with poly (A), and ligated to Illumina sequencing adapters. The size-selected fragments were amplified and purified. Sixteen libraries were sequenced by Illumina HiSeq™ 2500 at BGI (Shenzhen, China). RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2020-06-30
Run
Run accession Run data file information
File nameFile size (MB)
CRR058002 CRR058002_f1.fq.gz
CRR058002_r2.fq.gz
384.58
463.97
SubmitterBiyan Zhou (zhoulabscau2@163.com)
OrganizationSouth China Agricultural University
Date submitted2019-07-04