Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted using RNAprep Pure Plant Kit (Polysaccharides & Polyphenolics-rich) (Tiangen Biotech, China). For RNA-seq library construction, 5?g of total RNA with appropriate amount of Oligo-dT25 beads (Invitrogen, USA) was used. The mRNA was fragmented into short fragments, and reversed transcribed into cDNA by random primers. Second-strand cDNA was synthesized using DNA polymerase I, RNase H, dNTPs. After that, the cDNA fragments were purified by Agencourt AMPure XP (Beckman Coulter, USA). The purified cDNA fragments were end repaired, added with poly (A), and ligated to Illumina sequencing adapters. The size-selected fragments were amplified and purified. Sixteen libraries were sequenced by Illumina HiSeq™ 2500 at BGI (Shenzhen, China). |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|