Experiment information
Accession CRX051220
Organism Eriobotrya japonica
Title The clean reads in the next generation sequencing of loquat
BioProject PRJCA001507
BioSample SAMC071464
Platform Illumina HiSeq X Ten
Library name Construction protocol Strategy Source Selection Layout
High-quality genomic DNA was randomly interrupted by ultrasound. DNA fragments ranging from 200~500 bp were recovered by electrophoresis. T4 DNA Polymerase, Klenow DNA Polymerase and T4 PNK were used to repair cohesive ends to blunt ends. The 3′ end of DNA fragment was amended including an “A” base and ligated to adaptors, including a 5′ end T base. All fragments were recovered by electrophoresis and then paired-end sequenced by an Illumina HiSeq X Ten (San Diego, CA, USA). The adaptors and low-quality reads with more than 20% bases of quality value ≤10 in raw sequence data in FASTQ format were filtered by Trimmomatic. Only clean reads were used in the subsequent analysis. WGA GENOMIC unspecified PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 500
Release date2019-06-24
Run accession Release date Run data file information
File nameFile size (MB)
CRR056810 2019-06-24 Unknown_K703-01-R01_good_1.fq.gz
SubmitterShuang Jiang (271339616@qq.com)
OrganizationsShanghai Academy of Agricultural Sciences
Date submitted2019-06-17
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